Leila Saadat Aldaghi; Hasan Rezaee Seresht; Hamid Cheshomi
Volume 23, Issue 2 , March and April 2016, , Pages 353-359
Abstract
Background & Objectives: Breast cancer is the most common cancer affecting women so that it is the second common cancer (after lung cancer) in women. Vitex pseudo negundo is used as a traditional medicine. Recently, the biological activities of Vitex pseudo negundo plants have been reported as possessing ...
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Background & Objectives: Breast cancer is the most common cancer affecting women so that it is the second common cancer (after lung cancer) in women. Vitex pseudo negundo is used as a traditional medicine. Recently, the biological activities of Vitex pseudo negundo plants have been reported as possessing anticancer, antibacterial, antiulcer and antifungal properties. However, the antitumor effects of this medicine have not been studied in cancer cell lines. The aim of this study was to investigate the antitumor effect of Vitex pseudo negundo fruit on breast cancer cell lines. Materials & Methods: Breast cancer (MCF7) cell lines were cultured in DMEM medium. Vitex pseudo negundo fruit was extracted; and different dilutions of Vitex pseudo negundo extract (5mg/mL to 100mg/mL) were added to cell culture. Cell viability was quantitated by MTT assay after 72 hours. Results: The findings indicate that the extract of Vitex pseudo negundo fruit on MCF7 cancer cell lines had cytotoxicity in all concentrations and the highest inhibition was 50 and 100 mg/ml concentrations. Conclusion: Our study shows that Vitex pseudo negundo fruit extract has cytotoxic effects on tumor cells, It seems that Vitex pseudo negundo fruit could be considered as a promising chemotherapeutic agent in cancer treatment.
Bagher Seyyed Alipour; Moghaddeseh Fazeli; Salman Ahmadi Asb Chin; Hamid Cheshomi; Leila Saadat Aldaghi
Volume 22, Issue 5 , November and December 2015, , Pages 919-926
Abstract
Background and objective: Chalcones are belonged to an important group of flavonoids which has proven anti-inflammatory and antineoplastic effects. Assessing cellular Cytotoxicity effect of new synthetic chalcone on HeLa cell line is the aim of present study. Materials and Methods: In this experimental ...
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Background and objective: Chalcones are belonged to an important group of flavonoids which has proven anti-inflammatory and antineoplastic effects. Assessing cellular Cytotoxicity effect of new synthetic chalcone on HeLa cell line is the aim of present study. Materials and Methods: In this experimental in vitro study, HeLa cells were treated with various concentrations of chalcone for 72 hours and cell death rates was evaluated by MTT test. Results were analyzed by Student T-Test and morphological changes were assessed by Acridine orange and propidium iodide (PI) staining. Results: Our results showed that new synthetic chalcone in studied concentrations inhibited tumor cell growth. Highest percent (50.44%) of growth inhibition was seen in 40 µg/ml concentration and after 72 hours IC50 was 40µg/ml. morphological changes of HeLa cells after 24 hours were also strengthened the probability of cellular apoptosis. Conclusion: The results showed that new chalcone has inhibitive effects on HeLa tumor cell line and a probable mechanism of apoptosis can causes cellular death.